If you have any problems related to the accessibility of any content (or if you want to request that a specific publication be accessible), please contact us at scholarworks@unr.edu.
Author | Amaral, Paloma de Melo | |
Author | Silva Mariz, Lays Debora | |
Author | Benedeti, Pedro Del Bianco | |
Author | da Silva, Lorrayny G. | |
Author | de Paula, Eduardo M. | |
Author | Monteiro, Hugo F. | |
Author | Shenkoru, Teshome | |
Author | Santos, Stefanie A. | |
Author | Poulson, Simon R. | |
Author | Faciola, Antonio P. | |
Date Accessioned | 2019-08-14T00:25:36Z | |
Date Available | 2019-08-14T00:25:36Z | |
Date of Issue | 2016 | |
Identifier (Citation) | Amaral PdM, Mariz LDS, Benedeti PDB, Silva LGd, Paula EMd, Monteiro HF, et al. (2016) Effects of Static or Oscillating Dietary Crude Protein Levels on Fermentation Dynamics of Beef Cattle Diets Using a Dual-Flow Continuous Culture System. PLoS ONE 11(12): e0169170. https://doi.org/10.1371/journal.pone.0169170 | en_US |
ISSN | 1932-6203 | |
Identifier (URI) | http://hdl.handle.net/11714/5845 | |
Description | The objective of this study was to evaluate the effects of increasing dietary crude protein (CP) levels and also comparing the effects of static versus oscillating dietary CP on ruminal nutrient digestibility, ruminal fermentation, nitrogen (N) metabolism, and microbial efficiency in beef cattle diets using a dual-flow continuous culture system. Eight fermenters (1,223 + 21 mL) were used in a replicated 4 x 4 Latin square design with periods lasting 12 d each (8 d for adaptation and 4 d for sampling). Dietary treatments were: 1) 10% CP, 2) 12% CP, 3) 14% CP, and 4) 10 and 14% CP diets oscillating at 48-h intervals. Experimental diets consisted of 50% orchard hay and 50% concentrate. Fermenters were fed 72 g/d and solid and liquid dilution rates were adjusted to 5.5 and 11%/h, respectively. Data were analyzed using the MIXED procedure in SAS with alpha = 0.05. Apparent and true ruminal digestibilities of dry matter and organic matter were not affected (P > 0.05) by increasing dietary CP, nor by oscillating dietary CP. Total volatile fatty acids concentration and molar proportions of acetate, propionate, butyrate, valerate, iso-butyrate and iso-valerate were not affected (P > 0.05) by increasing or oscillating dietary CP. Ruminal NH3-N concentration increased linearly (P < 0.01) in response to increasing dietary CP. Total N, non-ammonia N, and rumen undegraded protein flows did not differ among treatments or between oscillating dietary CP and static 12% CP. Microbial N and NH3-N flows and microbial efficiency did not differ when comparing oscillating versus static CP (P > 0.05). However, there was a quadratic effect (P < 0.05) for these variables when dietary CP was increased. These results indicate that either ruminal microorganisms do not respond to oscillating CP levels or are capable of coping with 48-h periods of undernourishment. | en_US |
Language | en_US | en_US |
Publisher | Plos One | en_US |
Rights | Attribution 4.0 International | * |
Rights URL | http://creativecommons.org/licenses/by/4.0/ | * |
Title | Effects of Static or Oscillating Dietary Crude Protein Levels on Fermentation Dynamics of Beef Cattle Diets Using a Dual-Flow Continuous Culture System | en_US |
Type | Article | en_US |
Rights Holder | Authors | en_US |
Identifier (DOI) | 10.1371/journal.pone.0169170 | en_US |